Composite

Part:BBa_J100001

Designed by: Eric Sawyer   Group: Campbell M Lab   (2011-05-24)


pTet+RBS+Cre2SAT1Clause+pLpp+tRNA CCACU

Suppressor tRNA CCACU (K199002) is produced under regulation by the lpp promoter (K199106). The tRNA suppresses one of the frameshift mutations in Cre, restoring its function.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 468
    Illegal BamHI site found at 1215
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 154
    Illegal AgeI site found at 1252
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None